Fig. 8: The Refractive Index of Immersion Oil

Immersion oil used for microscopy has the same refractive index as glass, meaning it behaves optically as if it was glass. This is a bottle of immersion oil with the dropper filled with oil. Both the glass and the oil have a refractive index of 1.52 and the dropper becomes almost invisible.

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Microbiology Laboratory Manual by Gary E. Kaiser, PhD, Professor of Microbiology
is licensed under a Creative Commons Attribution 4.0 International License.
Last updated: August, 2017