Fig. 20: Steaming the Slide

Holding the slide with a test tube holder, carefully heat the slide in the flame of a bunsen burner until the stain just begins to steam. Remove the slide from the heat until steaming stops; then gently reheat. Continue steaming the smear in this manner for five minutes. As the malachite green evaporates, continually add more. Do not let the paper dry out.


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